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All HF-restriction enzymes come with Gel Loading Dye, Purple (6X). New England Biolabs single cutting restriction noti hf enzyme Single Cutting Restriction Noti Hf Enzyme, supplied by New England Biolabs, used in various techniques. However, this research should always be done in safe and ethical manner. NotI-HF, NEB has the same specificity as NotI, but has been engineered for significantly reduced star activity and performance in a single buffer ( CutSmart Buffer ). New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA. We are excited to announce that all reaction buffers are now BSA-free. This product is intended for research purposes only. BamHI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot 10133983. While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.įor more information about commercial rights, please email us at. This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin). Reaction mixtures included 1 µg of DNA and 1 µL of Anza. Plasmid DNA (6,215 bp) was digested using Anza restriction enzymes 3 BcuI and 47 Eco521, as well as Competitor N SpeI-HF (isoschizomer to BcuI) and Competitor N EagI-HF (isoschizomer to Eco52I). The following file naming structure is used to name these document files: _ XbaI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot 10128088. Anza restriction enzymes are formulated to complete digestion in just 15 minutes. The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot.